1. Injongo: Le nkqubo ijolise ekunikezeleni inkqubo emiselweyo yemisebenzi ye-ASEptic kunye nokukhuselwa kwamagumbi amdaka.
2. Ubungakanani besicelo: ILebhu yokuvavanya i-biologiz
3. Umntu onoxanduva: I-QC Supervisor Tester
4.Definition: Akukho
5. Amanyathelo okhuseleko
Ngokungqongqo ukwenza imisebenzi ye-aptic ukukhusela ungcoliseko lwe-rotial; Abaqhubi kufuneka bacime isibane ngaphambi kokungena kwigumbi lokuphumla.
6.Usa
6.1. Igumbi le-Sterle kufuneka lixhotywe ngegumbi lokusebenza le-Sterle kunye negumbi le-buffer. Ukucoceka kwegumbi lokusebenza leSteria kufuneka kufikelele kwiklasi i-10000. Iqondo lobushushu lifanele ligcinwe kwi-20-20 ° C kwaye umswakama kufuneka ugcinwe nge-45-60%. Ukucoceka kwebhentshi ecocekileyo kufuneka kufikelele kwiklasi ye-100.
6.2. Igumbi le-Sterle kufuneka ligcinwe licocekile, kwaye kunqatshelwe ngokungqongqo ukufaka inkunkuma ukukhusela ungcoliseko.
6.3. Ngokuthintela ngokungqongqo kwazo zonke izixhobo zeSertilization kunye neNkcubeko. Abo bangcolisekileyo kufuneka bayeke ukuzisebenzisa.
6.4. Igumbi eliyinqwanqwa kufuneka lixhotyiswe ngabathengisi bokusebenza, njenge-5% yesisombululo seCres, i-70 ye-70% yotywala, i-0.1% chlomethine isisombululo, njl.
6.5. Igumbi eliyinqwanqwa kufuneka lincitshiswe rhoqo kwaye licocwe ngesibulala-ntsholongwane olufanelekileyo ukuqinisekisa ukuba ukucoceka kwegumbi le-Sterile kuyahlangabezana neemfuno.
6.6. Zonke izixhobo, izixhobo, izitya kunye nezinye izinto ezifuna ukungeniswa kwinqanaba le-Sterle kufuneka zisongelwe ngokuqinileyo kwaye zifakwe kwiindlela ezifanelekileyo.
6.7. Phambi kokuba ungene kwigumbi lokuphumla, abasebenzi kufuneka bahlambe izandla zabo ngesepha okanye intsholongwane, kwaye emva koko batshintshe impahla ekhethekileyo yomsebenzi, izihlangu, ii-hats kunye ne-roble kwigumbi le-buffer (okanye i-ethanol) ngaphambi kokungena kwigumbi lokuphumla. Yenza imisebenzi kwi-Bactery Chamber.
6.8. Ngaphambi kokusebenzisa igumbi eliyinqindi, isibane se-ultraviolet segumbi elinodaka kufuneka sivulelwe i-inradication kunye nokucofa imizuzu engaphezulu kwemizuzu engama-30, kwaye ibhentshi ecocekileyo kufuneka ivulwe kumoya ovuthuzayo ngaxeshanye. Emva kokugqitywa kwephulo, igumbi elingenanto kufuneka licocwe emva kwexesha kwaye emva koko ifakwe i-ultraviolet yokukhanya kwimizuzu engama-20.
6.9. Ngaphambi kokuba kuhlolwe, ukupakishwa kwangaphandle kwesampulu yovavanyo kufuneka igcinwe ingenzi i-isti yenzekile kwaye akufuneki ivulwe ukuthintela ungcoliseko. Ngaphambi kokuhlolwa, sebenzisa i-70% yeebhola zekotoni zekotoni zokubulala intsholongwane.
6.10. Ngexesha lokusebenza nganye, kulawulo olubi kufuneka kwenziwe ukukhangela ukuthembeka komsebenzi we-ASEptic.
6.11. Xa ifumana ulwelo lwentsholongwane, kufuneka usebenzise ibhola yokubethelwa ukuze uyifumane. Sukuchukumisa umququmbene nomlomo wakho.
6.12. Inale ye-inoculation kufuneka ifakwe ilangatye ngedangatye ngaphambi nasemva kwesinye. Emva kokupholisa, inkcubeko inokuchithwa.
6.13. Imigca, iityhubhu zovavanyo, izitya ze-petri kunye nezinye iimpahla eziqukethe ulwelo lwe-bacterial kufuneka zifakwe kwibhakethi ye-wilterial equlethe isisombululo se-5% yeLyson ukuze ikhutshelwe iintsholongwane emva kweeyure ezingama-24.
6.14. Ukuba kukho ulwelo lwe-bacterial oluchithiwe kwitafile okanye phantsi, kuya kufuneka uphinde ugalele isisombululo se-5% ye-carbolic i-scowol okanye i-3% lysol kwindawo engama-30 ubuncinci ngaphambi kokuyinyanga. Xa iimpahla zomsebenzi kunye nemikhono zingcolisiwe ngulwelo lwentsholongwane, kufuneka zikhutshelwe kwangoko kwaye zihlanjwe emva kokutshutshiswa okuphezulu.
6.15. Zonke izinto ezineebhaktiriya eziphilayo kufuneka zibulale intsholongwane phambi kokuba iphulwe phantsi kwetephu. Ithintelwe ngokungqongqo ukuba ingcolise umjelo wamanzi amdaka.
6.16. Inani leekoloni kwinqanaba le-Stele kufuneka lihlolwe ngenyanga. With the clean bench open, take a number of sterile petri dishes with an inner diameter of 90 mm, and aseptically inject about 15 ml of nutrient agar culture medium that has been melted and cooled to about 45°C. Emva kokuqina, yibeka ecaleni kwe-30 ukuya kwi-35 ifakwe iiyure ezingama-48 kwi-a incubator. Emva kokungqina ukungathobeli, thatha iipleyiti ezi-3 ukuya kwezi-5 kwaye ubeke ngasekhohlo, phakathi nasekunene kwendawo yokusebenza. Emva kokuvula isiciko kwaye sizivelise imizuzu engama-30, zibeka ezantsi ukuya kwi-30 ukuya kwi-35 ° C I-C I-CUUBATOtor iiyure ezingama-48 kwaye zikhuphe. vavanya. The average number of miscellaneous bacteria on the plate in a class 100 clean area shall not exceed 1 colony, and the average number in a class 10000 clean room shall not exceed 3 colonies. Ukuba umda ugqithile, igumbi elinemifanekiso kufuneka ibulale ngokucokisekileyo kude kube kuhlolwa okuphindaphindiweyo kufezekisa iimfuno.
7. Jonga kwisahluko (indlela yokuhlola i-staility) kwiindlela zokuhlola iziyobisi "kunye" ne-chinaragement china esemgangathweni yokusebenza kweziyobisi ".
8. ISebe lokuHambisa: iSebe loLawulo lweMigangatho
Igumbi lokucoca igumbi lokucoca igumbi:
Emva kokufumana imeko ye-STRLEF kunye neZixhobo eziNzulu, kufuneka sigcine i-State State ukuba sifunde i-Microorganism eyaziwayo okanye isebenzise imisebenzi yawo. Ngaphandle koko, imicroorganisosms ezahlukeneyo ukusuka ngaphandle inokuxuba ngokulula. I-phenomenon yokuxuba kwe-microorganisms engaphandle ibizwa ngokuba yintsholongwane kwi-microbiology. Ukuthintela ungcoliseko yindlela ebalulekileyo yomsebenzi we-microbiological. Gcwalisa i-sterilizas epheleleyo kwisandla esinye kunye nokuthintela ungcoliseko kwezinye izinto zimbini zendlela ye-ASEptic. Ukongeza, kufuneka sithintele i-microorganisms phantsi kwesifundo, ngakumbi i-microorganisms ye-pitogenism okanye i-microorganisos ye-generganisment engekhoyo kwindalo, ukubaleka kwizikhongozeli zethu zokulinga kwindawo yangaphandle. Ngezi njongo, kwi-microbiology, zininzi iindlela.
Igumbi eliyi-Stele lihlala ligumbi elincinci elimiselweyo kwiLebhu ye-microbiology. Inokwakhiwa ngamashiti kunye neglasi. Indawo ayifanele ibe mkhulu kakhulu, malunga ne-4-5 isikwere, kwaye ukuphakama kufanele kube malunga ne-2 yeemitha. Igumbi le-buffer kufuneka libekwe ngaphandle kwegumbi elingaphandle. Umnyango wegumbi le-buffer kunye nomnyango wegumbi lokungena ngaphandle kwegumbi akufuneki ukuba lijonge kwicala elinye ukuthintela inqwelo moya ukuzisa intsholongwane yeengxaki. Zombini igumbi elingenanto kunye negumbi le-buffer kufuneka libe ngumoya. Izixhobo ze-Indoor Izixhobo zomoya kufuneka zibe nezixhobo zomoya. Umgangatho kunye neendonga zegumbi elinodaka kufuneka zigudile, kunzima ukuzigcina ubumdaka kwaye kulula ukuzicoca. Umphezulu womsebenzi kufuneka ube kwinqanaba. Zombini igumbi elinobuqhetseba kunye negumbi le-buffer lixhotywe ngezibane ze-ultraviolet. Izibane ze-ultraviolet kwindawo enamagumbi ayi-1 kwimitha ukusuka kumphezulu womsebenzi. Abasebenzi abangena kwigumbi lokuphumla kufuneka banxibe iimpahla kunye neminqwazi esetyenziswayo.
Okwangoku, amagumbi anezinto ezimnandi ezikhoyo kwiifektri ze-microbiology, ngelixa iilebhu ngokubanzi zisebenzisa ibhentshi ecocekileyo. Umsebenzi ophambili webhentshi ecocekileyo kukusebenzisa isixhobo sokuhamba komoya se-laminar ukususa uthuli ngeendlela ezahlukeneyo kubandakanya i-microorganisms kumgangatho womsebenzi. Isixhobo sombane sivumela umoya ukuba udlule kwi-hep filter kwaye ufaka umphezulu womsebenzi, ukuze umphezulu womsebenzi uhlala ugcinwe phantsi kolawulo lomoya oqukuqelayo. Ngaphezu koko, kukho ikhethini elihamba ngesantya esiphezulu kwicala elisondeleyo ngaphandle kokuthintela umoya ongaphandle ukuba ungangeni.
Kwiindawo ezinemeko ezinzima, iibhokisi ze-wistern ezinamaplanga nazo zinokusetyenziswa endaweni yokucoca ibhentshi. Ibhokisi ye-Sterle inolwakhiwo olulula kwaye kulula ukushukuma. Kukho imingxunya emibini phambi kwebhokisi, evalwe ngeengcango-ukutsala iingcango xa zingasebenzi. Ungandindisa iingalo zakho ngexesha lokusebenza. Inxalenye ephezulu yomphambili ngaphambili ixhotyiswe ngeglasi ukuba iququzelele ukusebenza ngaphakathi. Kukho isibane se-ultraviolet ngaphakathi kwebhokisi, kwaye izixhobo zempahla kunye nebacteria zinokubekwa kumnyango omncinci ecaleni.
Iindlela zokusebenza ezisebenzayo ze-ASEptige azidlali kuphela indima ebaluleke kakhulu kuphando lwe-Microbiological kunye nezicelo, kodwa zisetyenziswa ngokubanzi kwii-biotechnologies ezininzi. Umzekelo, itekhnoloji yeTransgenic, i-monoclonnika i-anticlon
IXESHA LOKUQALA: Mar-06-2024