1. Injongo: Le nkqubo ijolise ekuboneleleni ngenkqubo eqhelekileyo yokusebenza kwe-aseptic kunye nokukhusela amagumbi angenazintsholongwane.
2. Ububanzi besicelo: ilabhoratri yovavanyo lwebhayoloji
3. Umntu onoxanduva: Umphathi weQC Umvavanyi
4. Inkcazo: Akukho
5. Amanyathelo okhuseleko
Yenza imisebenzi yokungabolisi iintsholongwane ngokungqongqo ukuze uthintele ungcoliseko lweentsholongwane; abaqhubi kufuneka bacime isibane se-UV ngaphambi kokuba bangene kwigumbi elinokungcola.
6. Iinkqubo
6.1. Igumbi elicocekileyo kufuneka lixhotyiswe ngegumbi lokuhlinza elicocekileyo kunye negumbi lokukhusela. Ukucoceka kwegumbi lokuhlinza elicocekileyo kufuneka kufikelele kwiklasi ye-10000. Ubushushu bangaphakathi kufuneka bugcinwe kwi-20-24°C kwaye ukufuma kufuneka kugcinwe kwi-45-60%. Ukucoceka kwebhentshi ecocekileyo kufuneka kufikelele kwiklasi ye-100.
6.2. Igumbi elingenazintsholongwane kufuneka ligcinwe licocekile, kwaye akuvumelekanga ngokungqongqo ukuqokelela inkunkuma ukuze kuthintelwe ungcoliseko.
6.3. Kuthintelwe ngokungqongqo ukungcoliswa kwazo zonke izixhobo zokubulala iintsholongwane kunye nezixhobo zokucoca. Ezo zingcolisiweyo maziyeke ukuzisebenzisa.
6.4. Igumbi elicocekileyo kufuneka lixhotyiswe ngezibulali-ntsholongwane zoxinzelelo olusebenzayo, ezifana nesisombululo se-cresol esingu-5%, i-70% yotywala, isisombululo se-chlormethionine esingu-0.1%, njl.
6.5. Igumbi elingenazintsholongwane kufuneka lihlanjululwe rhoqo kwaye licocwe ngesibulali-ntsholongwane esifanelekileyo ukuqinisekisa ukuba ukucoceka kwegumbi elingenazintsholongwane kuyahlangabezana neemfuno.
6.6 Zonke izixhobo, izixhobo, izitya kunye nezinye izinto ekufuneka zingeniswe kwigumbi elingcolileyo kufuneka zisongwe kakuhle kwaye zicocwe ngeentsholongwane ngeendlela ezifanelekileyo.
6.7. Ngaphambi kokuba bangene kwigumbi elingenazintsholongwane, abasebenzi kufuneka bahlambe izandla zabo ngesepha okanye isibulali-ntsholongwane, baze batshintshe iimpahla zomsebenzi ezikhethekileyo, izihlangu, iminqwazi, iimaski kunye neeglavu kwigumbi elinomoya ococekileyo (okanye basule izandla zabo kwakhona nge-70% ethanol) ngaphambi kokuba bangene kwigumbi elingenazintsholongwane. Yenza utyando kwigumbi elingenazintsholongwane.
6.8. Ngaphambi kokusebenzisa igumbi elicocekileyo, isibane se-ultraviolet kwigumbi elicocekileyo kufuneka sivulwe ukuze sikhanyise kwaye sibulale iintsholongwane kangangemizuzu engaphezu kwama-30, kwaye ibhentshi ecocekileyo kufuneka ivulwe ukuze umoya uvuthe ngaxeshanye. Emva kokuba umsebenzi ugqityiwe, igumbi elicocekileyo kufuneka licocwe ngexesha kwaye emva koko lihlanjululwe ngokukhanya kwe-ultraviolet imizuzu engama-20.
6.9. Ngaphambi kokuhlolwa, iphakheji yangaphandle yesampulu yovavanyo kufuneka igcinwe icocekile kwaye akufuneki ivulwe ukuthintela ungcoliseko. Ngaphambi kokuhlolwa, sebenzisa iibhola zekotoni ezingama-70% zotywala ukubulala iintsholongwane kumphezulu ongaphandle.
6.10. Ngexesha lomsebenzi ngamnye, kufuneka kwenziwe ulawulo olungalunganga ukujonga ukuthembeka komsebenzi we-aseptic.
6.11. Xa ufunxa ulwelo oluyintsholongwane, kufuneka usebenzise ibhola yokufunxa ukuze ulufunxe. Musa ukuyichukumisa ngqo ingca ngomlomo wakho.
6.12. Inaliti yokugonya kufuneka ihlanjululwe ngamalangatye ngaphambi nasemva kokusetyenziswa ngakunye. Emva kokupholisa, i-culture ingagonywa.
6.13. Iistraw, iityhubhu zokuvavanya, izitya zepetri kunye nezinye izixhobo ezine-bacterial liquid kufuneka zifakwe kwibhakethi yokubulala iintsholongwane equlethe isisombululo se-5% seLysol ukuze kubulawe iintsholongwane, kwaye zikhutshwe zihlanjwe emva kweeyure ezingama-24.
6.14. Ukuba kukho ulwelo lwebhaktiriya oluchithekileyo etafileni okanye phantsi, kufuneka ngoko nangoko ugalele isisombululo se-5% se-carbolic acid okanye i-3% yeLysol kwindawo engcolileyo ubuncinane imizuzu engama-30 ngaphambi kokuba uyinyange. Xa iimpahla zomsebenzi kunye neminqwazi ingcoliswe lulwelo lwebhaktiriya, kufuneka ikhutshwe ngoko nangoko kwaye ihlanjwe emva kokucoca ngomphunga ngoxinzelelo oluphezulu.
6.15. Zonke izinto ezineentsholongwane eziphilayo kufuneka zibulawe iintsholongwane ngaphambi kokuba zihlanjwe phantsi kwempompo. Akuvumelekanga ngokupheleleyo ukungcolisa umjelo wamanzi amdaka.
6.16. Inani leekoloni kwigumbi elicocekileyo kufuneka lihlolwe nyanga zonke. Xa ibhentshi ivuliwe, thatha inani leesitya ze-petri ezicocekileyo ezinobubanzi obungaphakathi obuyi-90 mm, kwaye ufake i-15 ml ye-nutrient agar medium enyibilikisiweyo yaze yapholiswa ukuya kuma-45°C. Emva kokuqina, yibeke phantsi kwi-30 ukuya kuma-35 Incubate iiyure ezingama-48 kwi-℃ incubator. Emva kokubonisa ukuba ayicocekanga, thatha iipleyiti ezi-3 ukuya kwezi-5 uze uzibeke ngasekhohlo, embindini nasekunene kwindawo yokusebenza. Emva kokuvula isigqubuthelo kwaye uzibeke imizuzu engama-30, zibeke phantsi kwi-30 ukuya kuma-35°C incubator iiyure ezingama-48 uze uzikhuphe. jonga. Inani eliqhelekileyo leebhaktheriya ezahlukeneyo kwipleyiti kwindawo ecocekileyo yeklasi ye-100 akufuneki lidlule ikholoni e-1, kwaye inani eliqhelekileyo kwigumbi elicocekileyo leklasi ye-10000 akufuneki lidlule iikholoni ezi-3. Ukuba umda udlulile, igumbi elicocekileyo kufuneka licocwe ngokupheleleyo de kube uhlolo oluphindaphindwayo luhlangabezana neemfuno.
7. Jonga isahluko (iNdlela yoVavanyo lweeNtlungu) kwi "Drug Hygienic Inspection Methods" kunye ne "China Standard Operating Practices for Drug Inspection".
8. ISebe loSasazo: ISebe loLawulo loMgangatho
Isikhokelo sobugcisa segumbi lokucoca:
Emva kokufumana indawo engcolileyo kunye nezinto ezicocekileyo, kufuneka sigcine imeko ecocekileyo ukuze sifunde i-microorganism ethile eyaziwayo okanye sisebenzise imisebenzi yayo. Ngaphandle koko, ii-microorganism ezahlukeneyo ezivela ngaphandle zinokudibana ngokulula. Imeko yokuxuba ii-microorganisms ezingabalulekanga ezivela ngaphandle ibizwa ngokuba yi-contaminating bacteria kwi-microbiology. Ukuthintela ungcoliseko yindlela ebalulekileyo kumsebenzi we-microbiology. Ukucoca ngokupheleleyo kwelinye icala kunye nokuthintela ungcoliseko kwelinye icala ziinkalo ezimbini zendlela ye-aseptic. Ukongeza, kufuneka sithintele ii-microorganisms ezifundwayo, ngakumbi ii-microorganisms ezibangela izifo okanye ii-microorganisms ezenziwe ngemfuza ezingekhoyo kwindalo, ukuba zingaphumi kwizikhongozeli zethu zovavanyo ziye kwindawo yangaphandle. Ngezi njongo, kwi-microbiology, kukho amanyathelo amaninzi.
Igumbi elicocekileyo lidla ngokuba ligumbi elincinci elicwangciswe ngokukodwa kwilebhu ye-microbiology. Lingakhiwa ngamashiti neglasi. Indawo akufuneki ibe nkulu kakhulu, malunga ne-4-5 square metres, kwaye ukuphakama kufuneka kube malunga ne-2.5 metres. Igumbi elicocekileyo kufuneka licwangciswe ngaphandle kwegumbi elicocekileyo. Ucango lwegumbi elicocekileyo kunye nocango lwegumbi elicocekileyo akufuneki lujonge kwicala elifanayo ukuthintela ukuhamba komoya ekungeniseni iintsholongwane ezahlukeneyo. Zombini igumbi elicocekileyo kunye negumbi elicocekileyo kufuneka zingangeni moya. Izixhobo zokungenisa umoya zangaphakathi kufuneka zibe nezixhobo zokucoca umoya. Umgangatho kunye neendonga zegumbi elicocekileyo kufuneka zibe bushelelezi, kube nzima ukugcina ukungcola kwaye kube lula ukucoca. Umphezulu womsebenzi kufuneka ube kumgangatho othe tyaba. Zombini igumbi elicocekileyo kunye negumbi elicocekileyo zixhotyiswe ngezibane ze-ultraviolet. Izibane ze-ultraviolet kwigumbi elicocekileyo zikude ngemitha e-1 ukusuka kumphezulu womsebenzi. Abasebenzi abangena kwigumbi elicocekileyo kufuneka banxibe iimpahla ezicocekileyo kunye neminqwazi.
Okwangoku, amagumbi angenazintsholongwane akhona kakhulu kwiifektri ze-microbiology, ngelixa iilabhoratri eziqhelekileyo zisebenzisa ibhentshi ecocekileyo. Umsebenzi ophambili webhentshi ecocekileyo kukusebenzisa isixhobo sokuhamba komoya esincinci ukususa uthuli oluncinci oluquka iintsholongwane kumphezulu womsebenzi. Esi sixhobo sombane sivumela umoya ukuba udlule kwisihluzo se-hepa uze ungene kumphezulu womsebenzi, ukuze umphezulu womsebenzi uhlale ulawulwa ngumoya ongenayo izintsholongwane. Ngaphezu koko, kukho ikhethini yomoya ekhawulezayo ecaleni elikufutshane nangaphandle ukuthintela umoya wangaphandle webhaktheriya ukuba ungangeni.
Kwiindawo ezineemeko ezinzima, iibhokisi zomthi ezicocekileyo zingasetyenziswa endaweni yebhentshi ecocekileyo. Ibhokisi ecocekileyo inesakhiwo esilula kwaye kulula ukuyihambisa. Kukho imingxunya emibini ngaphambili kwebhokisi, evalwe ziingcango zokutyhala xa zingasebenzi. Ungazolula iingalo zakho ngaphakathi ngexesha lokusebenza. Inxalenye ephezulu yangaphambili ixhotyiswe ngeglasi ukuze kube lula ukusebenza ngaphakathi. Kukho isibane se-ultraviolet ngaphakathi kwebhokisi, kwaye izixhobo kunye neentsholongwane zinokufakwa ngomnyango omncinci ecaleni.
Iindlela zokusebenza ze-Aseptic okwangoku azidlali indima ebalulekileyo kuphando lwe-microbiological kunye nokusetyenziswa kwayo, kodwa zikwasetyenziswa kakhulu kwiiteknoloji ezininzi ze-biotechnology. Umzekelo, iteknoloji ye-transgenic, iteknoloji ye-monoclonal antibody, njl.
Ixesha lokuthumela: Matshi-06-2024